Vijayakumar, N KRosamma, C AKAU2019-04-302019-04-301998171376http://krishikosh.egranth.ac.in/handle/1/5810101409PhDInvestigations to identify stable male sterile lines and better combiners for the exploitation of hybrid vigour in rice were conducted at College of Horticulture, Vetlantkkara and at Agricultural Research Station, Mannuthy during 1994-98. The study included evaluation of cytoplasmic - genic male sterile (CMS) lines to identify stable lines, identification of maintainers and restorers for different CMS lines, inheritance of fertility restoration, studies on the genetic distance between maintainers and restorers and genetic analysis of the hybrids. Out of the ten CMS lines evaluated, seven were identified as stable for pollen and spikelet sterility behaviour. Studies on the floral traits which influence outcrossing expressed variation between CMS lines and between seasons. Jyothi, Aruna, Pavizham and Ptb 10 produced more number of sterile hybrids when crossed with CMS lines having WA cytoplasm and hence these varieties can be used as maintainers. More number of fertile hybrid combinations were produced by Annapoorna, Matta Triveni, Kanchana, IR 36 and Aiswarya indicating the use of these varieties as restorers for WA cytoplasmic source. All the genotypes under study produced sterile hybrids when crossed with CMS lines having O. perennis cytoplasm and no restorers could be identified for this new source of cytoplasmic male sterility. Inheritance studies on fertility restoration indicated polygenic mode of inheritance for this particular character. Forty four rice genotypes included in the hybrid rice breeding programme were grouped into nine clusters, each having different number of genotypes. Maintainers and restorers were distributed in different clusters and no direct relation could be established between heterosis and genetic distance of the parents. Genetic analysis of the 34 fertile hybrids resulted in the identification of five hybrids having significant positive standard heterosis. Among the male sterile lines IR 68890A was identified as the best combiner for most of the agronomic traits and IR 68890A/Aiswarya was the best specific hybrid combination. Magnitude of the gca variance was more than the variance due to sca for days to flowering, total duration, harvest index, 100 seed weight and other grain characters indicating predominance of additive variance for these characters. Variance due to sca was more for plant height, tillers per plant, total dry matter production, grain yield per plant, straw yield per plant, panicle length and filled grains per panicle indicating predornlnance of variance due to dominance and epistasis. Studies on association of characters in hybrids revealed that total drymatter accumulation, harvest index, number of filled grains per panicle, grain breadth, panicle weight, grain density, flag leaf area and leaf area index at 60 days after sowing were positively correlated with grain yield produced by the hybrids. Negative influence of sterile cytoplasm was identified for most of the agronomic attributes.ennullIdentification of stable male sterile lines and better combiners for exploitation of hybrid vigour in rice (Oryza Sativa L.)Thesis