Verma, Pawan KumarMahajan, Lakshay2018-07-112018-07-112017No. of references 270http://krishikosh.egranth.ac.in/handle/1/5810058996The present study was aimed to evaluate the alterations in biochemical and oxidative biomarkers indicating hepatic, renal, testicular damage induced by the subacute exposure of imidacloprid and arsenic in male wistar rats. Rats (180-200 g) were divided into eight groups of six rats each and were subjected to various daily oral administrations for 28 days. Group I served as control, group II received imidacloprid @ 16.9 mg/kg b.wt by oral gavage, group III, IV and V received arsenic @ 50, 100 and 150 ppb orally in drinking water whereas group VI, VII and VIII received both imidacloprid and arsenic at different dose levels respectively. Total plasma proteins and albumin levels revealed a significant (P<0.05) fall in higher doses of arsenic as well as in combination groups while SGOT, SGPT, ACP, ALP, BUN and CR levels were increased significantly (P<0.05) in all treated groups as compared to the control. IMI and arsenic caused significant (P<0.05) elevation in MDA and AOPP whereas significant (P<0.05) decrease in TTH, GST, GR, GPx, SOD and CAT activities were observed in the blood, liver, kidney and testicular tissues. These finding were further confirmed by histological alterations in these tissues. In liver, mild to moderate degenerative changes were observed in IMI and arsenic administered rats. Arsenic administrations produce mild degenerative changes of renal tubular epithelium in a dose dependent manner. Microscopic examination of testes presented mild edematous fluid accumulation in interstitial spaces in the IMI administered group and arsenic exposure demonstrated mild degenerative changes in seminiferous tubules with the increasing doses of arsenic. Co-administration of IMI and arsenic produced more severe biochemical, antioxidant alterations in blood, liver, kidney and testes as compared to the individual administration of either toxicant in the wistar rats.ennullThesis