Yadav, Neelam R.Saroha, Vandanna2016-11-302016-11-302005http://krishikosh.egranth.ac.in/handle/1/88287The present study was conducted to develop micropropagation system in Aloe vera L. and establishment of the regenerated plantlets in the soil. Shoot tip and leaf explants from 4-5 cm long offshoots were collected from field grown plants. These explants were cultured on 18 different MS modified media fortified with different concentrations and combinations of auxin, cytokinins and additives. MS medium supplemented with TDZ (1.0 mg/l) produced highest shoot formation response and further upscaling was done on MS medium with BA (2.0 mg/l) and IAA (0.2 mg/l). For shoot elongation, Tyrosine (50.0 mg/l) alongwith BA (5.0 mg/l) and adenine sulphate (80 mg/l) in MS medium produced 158.5 per cent increase in shoot length along with rooting. Leaf explants did not respond to shoot formation, however, 24.9 per cent callusing was observed on medium with 2,4-D (1.0 mg/l). The regenerated shoots showed 100 per cent rooting in all the six media tried for rooting, but longest roots (9.16 cm) and highest number of roots per shoot (13.22) were observed on medium fortified with paclobutrazol (0.5 mg/l) after 45 days of transfer. The regenerated plants were kept for hardening in water overnight and transferred to the pots with soil and vermiculite mixture (1:1). The rooting medium also affected the survival rate of the regenerated plants in soil. Highest survival rate was observed in paclobutrazol rooted plantlets. The regenerated plants were analysed for homogeneity through SDS-PAGE and all the plants showed similar soluble protein banding pattern. Promising variant has been recorded among plants regenerated on paclobutrazol (0.5 mg/l) containing medium. It also contained around four times more soluble protein and one and a half times more sapogenin content than the field grown plants. It showed thin cuticle, succulent and broad leaves. These plants are under observation for their utility in gel extraction.enThesis