Kumari, ReenaJamra, Himani2023-02-242023-02-242021-06-29https://krishikosh.egranth.ac.in/handle/1/5810194464During present investigations highest PAL activity was found in seed (158.360±0.428 U/ml) and edible part (269.600±0.432 U/ml) of Phulle Super Bhagwa at 120 DAF. Arils of Kandhari Soft-502 was reported to have highest PAL activity (234.420±0.600 U/ml) at 120 DAF. The sequence of PAL gene isolated from the soft seeded Punica granatum variety Phulle super Bhagwa was of 509bp. The results of homology studies showed that the sequence of this gene was found to had 99.2% similarity with Punica granatum phenylalanine ammonia lyase mRNA. Phytochemical profiling of different varieties showed that maximum phenolic content was found in seed (9.810±0.021 GAE mg/g) and aril ((12.538±0.018 GAE mg/g) of Kandhari Soft-502. Highest flavonoid content was found in seed (10.682±0.032 QE mg/g fresh tissue) and aril (13.802±0.072 QE mg/g fresh tissue) of Kandhari Kabuli. Maximum antioxidant activity was found in seed ((83.986±0.258 ℅ DPPH scavenging ) of Kandhari Kabuli and aril ((118.896±0.252 ℅ DPPH scavenging ) of Kandhari soft-502. During present studies variation in pomological taits was observed in different varieties at different seed developmental stages. Maximum seed weight (18.564±0.035g) and TSS (17.218±0.087°B) was found in Punica granatum variety Kandhari Kabuli. The results of biochemical and molecular characterization of PAL at different developmental stages of seed showed that the expression of this gene was found to be more in soft seeded variety as compared to the hard seeded Punica granatum L. On the basis of the comparative analysis of PAL expression in both varieties it could be concluded that this is not specific gene for monolignol biosynthesis and might contribute to the seed softness trait.EnglishThesis