Rao, P.B.Pant, Bhaskar2021-07-072021-07-072021-01https://krishikosh.egranth.ac.in/handle/1/5810170193Traditional herbal medicines have always prevented numerous diseases which are fatal to the mankind. Synthetic drugs are frequently associated with several side effects. Thus, medicinal plants can be used as dependable natural alternatives. In the present study, antioxidants and in vitro anti-inflammatory potential of eight wild plant species of Lamiaceae in both aqua-ethanol and aqua acetone extracts was assessed by using standard methods. The extraction yield (%) was maximum in Pogostemon benghalensis (12.63) and Anisomeles indica (12.76) in aqua-ethanol and aqua-acetone, respectively. Majority of the phytochemicals were present in all the selected plant species except quinones and saponins which were absent in P. benghalensis. DPPH scavenging activity (%) was maximum in Micromeria biflora (75.89) in aqua-ethanol and Ocimum gratissimum (67.58) in aqua-acetone. Comparatively lower IC50 (μg/ml) values of DPPH were obtained in M. biflora (26.82) in aqua-ethanol than in O. gratissimum (64.81) in aqua-acetone. TAA (μg VCE/mg extract) was highest in Colebrookea oppositifolia (38.55) and M. biflora (34.96) in aqua-ethanol and aqua-acetone, respectively. Comparatively higher FRAP activity (μg BHTE/mg of extract) was exhibited by M. biflora in both aquaethanol and aqua-acetone (32.00 and 31.38, respectively) than in rest of the plant species. The TPC (μg GAE/mg extract) was maximum in O. gratissimum (18.07 and 18.43) in both aqua-ethanol and aqua-acetone, while maximum TFC (μg QE/mg extract) was present in C. oppositifolia (29.57±2.13 and 45.69±0.50) in both aqua-ethanol and aqua-acetone at 200 μg/ml of extract. Maximum inhibition (%) of albumin denaturation was observed in C. oppositifolia (58.66 and 47.43) in both aqua-ethanol and aquaacetone. Lowest IC50 (μg/ml) values were obtained in C. oppositifolia (72.17 and 133.98) in both aquaethanol and aqua-acetone indicating its higher anti-inflammatory potential. Positively strong correlation was exhibited in terms of DPPH, FRAP, TAA, and TPC by most of the plant species in both the solvents except in M. biflora, in which, it was very weak in aqua-acetone. Thus, among the solvents, aqua-ethanol extracts exhibited comparatively higher antioxidant potential than aqua-acetone and among the species, M. biflora, O. gratissimum and C. oppositifolia showed comparatively higher activity than in rest of species. Therefore, these plant species can be used as a natural plant antioxidants and can be exploited in pharmaceutical and nutraceutical industries.EnglishThesis