Suma MogaliRavikumar2021-09-182021-09-182016-08https://krishikosh.egranth.ac.in/handle/1/5810175915In the present investigation, eighty nine advanced breeding lines were evaluated for Mungbean Yellow Mosaic Virus (MYMV) resistance during two successive seasons summer2014 and summer 2015, amongst which, twenty fouradvanced lines showed moderate resistance to MYMV. Further, twenty four lines were evaluated in two replications for MYMV resistance during summer season 2015 under protected and unprotected condition. Out of twenty four advanced breeding lines, seventeen were found to be moderate resistant, three moderate susceptible, two susceptible and only one wild relatives of mungbean Vigna trilobata were found to be immune to MYMV during summerseason 2015.The genetic variability was also studied. Among the twenty four advanced breeding lines yield per plant were significantly and positively associated with number of pods per plant and number of seeds per pod. These advanced breeding lines which are high-yielding and also possess moderate resistance to MYMV can be released as new variety after large-scale field testing. The study was also conducted to validate molecular markers that were previously reported to be linked to major locus/QTLs governing MYMV resistance. Thirty markers were used for marker-trait analysis.Among the thirty,ten markers (viz.,CEDG008, CEDG257, CEDG006, CEDG244, CEDG115, CEDG041, CEDG084, CEDG201, CEDG304 and CEDG191) showed parental polymorphism. But these markers did not show association with MYMV resistance in advanced breeding lines. The crosses between MYMV susceptible and MYMV resistant parents were carried to develop mapping population for MYMV resistance. All the F1 plants from the cross between DGGV2 × Vigna trilobata were validated with thirty markers. Only two markers (CEDG006 and CEDG088) showed parental polymorphism in F1s. Two markers were used for molecular confirmation of F1 derived from a cross between DGGV2 × Vigna trilobata.EnglishThesis