BALRAM, MDNYANESHWAR B. DESHMUKH2020-10-312020-10-312019D10,396https://krishikosh.egranth.ac.in/handle/1/5810154013Kadiri 6 (K 6) is a popular high yielding, short duration groundnut variety possessing high oil and attractive kernel features, nevertheless K 6 is highly susceptible to late leaf spot (LLS) and rust diseases of groundnut. The present study sought to introgress LLS, rust resistance and high oleic acid traits from donor lines ICGVs 13193 and 15033 respectively into K 6 cultivar. Genotyping for target alleles was performed in first generation with gel based linked SSR markers and in later generations with high throughput Kompetitive allele specific PCR (KASP) based SNPs assay. Backcrossing and selfing resulted in the development 343 BC1F2s of which 16 plants were identified with homozygous alleles for rust, LLS and high oleic traits. Evaluation of these backcross progenies resulted in four progenies (BC1F2s-76, 216, 225, and 311) resistant to LLS and three progenies (BC1F2s-4, 186 and 327) resistant to rust. Morphoagronomic and nutritional quality analysis revealed three progenies (BC1F2s-76, 278 and 296) were superior for morpho-agronomic and nutritional traits over K 6. The pod features viz., pod beak, pod reticulation, pod constriction, and pod ridges were attractive over K 6. The higher oil content (>50.0%) was observed in 11 progenies (BC1F2s-5, 76, 120, 144, 186, 214, 225, 249, 278, 296 and 327) in comparison to K 6 (49.5%). The oleic acid was higher in three progenies BC1F2-76 (81.32%), BC1F2-278 (82.8%) and BC1F2-296 (83.4%). Author : DNYANESHWAR B. DESHMUKH Title of Thesis : MOLECULAR BREEDING FOR IMPROVEMENT OF FOLIAR FUNGAL DISEASE RESISTANCE AND OIL QUALITY IN GROUNDNUT (Arachis hypogaea L.) Degree : DOCTOR OF PHILOSOPHY Faculty : AGRICULTURE Discipline : MOLECULAR BIOLOGY AND BIOTECHNOLOGY Major advisor : Dr. M. BALRAM University : PROFESSOR JAYASHANKAR TELANGANA STATE AGRICULTURAL UNIVERSITY Year of submission : 2019 ABSTRACT These three progenies showed resistance to LLS a disease score of 3.0- 3.5 and rust resistance with a disease score of 4.0. The selected progenies featured with slight pod constriction and pod reticulation. Pod beak was slight in two progenies (BC1F2s-76 and 296) and medium in BC1F2-278. The pod ridges were absent in BC1F2s- 278 and 296 and slight in BC1F2-76. All the selected segregants showed higher oil content (>50.0%), oleic acid (>80.0%) and reduced linoleic acid (<10.0%). Advanced breeding lines derived from K 6 for LLS, rust resistance and high oleic traits will be suitable replacement for K 6 especially in disease hot spot locations.EnglishMOLECULAR BREEDING FOR IMPROVEMENT OF FOLIAR FUNGAL DISEASE RESISTANCE AND OIL QUALITY IN GROUNDNUT (Arachis hypogaea L.)Thesis