Senthilkumar, T.M.A.Parthiban, M.Ganesan, P.I.Nagalakshmi, K. ShrineRamadass, P.TANUVAS2019-12-302019-12-302014-01http://krishikosh.egranth.ac.in/handle/1/5810139005TNV_IVJ_Jan2014_91(01)_27-29Mycobacterium avium subsp. paratuberculosis (MAP) is the causal agent of Johne’s disease (JD), a chronic infectious enteritis of domestic and wild ruminants. Detection of MAP by PCR offers the advantage of reduced turnaround time compared with culture as well as PCR has been widely used as a diagnostic tool for detecting pathogens in sputum, stool, cerebrospinal fluids, blood and urine. Amplification of insertion element IS900 has proved to be highly sensitive and specific for detecting MAP strains (Collins et al., 1989).en-USVeterinary ScienceAnimal BiotechnologyArticle