LAKPALE, N.KESHARWANI, ASHRUTIKHARE, N.NANDA, H.C.CHANDRAKAR, G.TIWARI, P.K.2018-08-212018-08-212018http://krishikosh.egranth.ac.in/handle/1/5810066246The present investigation was carried out in the Dept. of Plant Pathology, College of Agriculture, IGKV, Raipur (C.G.) during 2017-18. Seed health evaluation was attempted for seed lots of different pea varieties viz. IPFD 10-12, Paras, Indira matar, KPMR 400, Shubhra, Ambika and local variety from randomly selected village. In dry seed examination, seed lots of different pea varieties showed distinct variation in healthy seeds, damaged, discoloured, small/under sized, shrunken seeds, weed seeds and inert matters. Washing test was also performed. Maximum spore load of associated seed borne mycoflora was observed in seed lot of local variety. Twelve seed borne mycoflora viz. Aspergillus flavus, Aspergillus niger, Aspergillus fumigatus, Trichoderma sp., Penicillium sp., Alternaria sp., Chaetomium sp., Curvularia sp., Fusarium sp., Basidiophora sp., Rhizoctonia sp. and Rhizopus sp. were detected and identified from the seeds of seven pea varieties by using standard blotter, agar plate, rolled paper towel and deep freeze method. Maximum percentage of mycoflora with reduced germination percentage was recorded from the seed lots of local variety whereas Shubhra variety harbours minimum percentage of mycoflora with higher germination percentage. Seedling symptom test was also performed by water agar test tube method and found the normal intact seedlings from the seeds inoculated with Trichoderma sp. and Penicillium sp., attributing the plant growth promoting properties. Agar plate method was most efficient in detecting seed borne mycoflora among all other incubation methods. Effect of isolated seed borne fungi was also tested for seedling vigour under seedling symptoms in pot culture condition by using seed and soil inoculation techniques. Majority of the detected seed borne mycoflora reduced the seedling vigour index and some were not. Two seed borne mycoflora of pea seeds i.e. Rhizopus sp. and Fusarium sp.were found pathogenic and seed to plant transmissible in nature whereas increased seedling vigour of pea varieties were recorded due to growth promoting ability of Trichoderma sp. Seed lots were treated with fungicides and biocontrol agent (Trichoderma+ vermicompost) and observed increased seedling vigour index as compare to untreated ones. After six months storage of seed lots, electrolyte leakage of pea seeds were recorded in terms of electrical conductivity and pH value of seed leachate. Due to the presence of seed borne mycoflora the EC value was increased and corresponding pH values were found inversely proportion.ennullThesis