S. AnoopTHAJUNNISA A S2020-09-172020-09-172019https://krishikosh.egranth.ac.in/handle/1/5810151434The study was undertaken to evaluate the efficacy of decellularised bovine omentum alone and in combination with mitomycin-C for the management of corneal injuries in dogs. All the cases with corneal injuries which subsequently lead to corneal ulceration were screened and 12 corneas were selected from 11 animals irrespective of breed, age and sex. Out of eleven dogs selected, 5 were Chinese Pug, 3 were French Bulldog, 2 were Pitbull and one was a Labrador Retriever. The age of affected animals ranged from 3 months to 38 months with mean value of 13.75 ± 3.09 months and 6 were male and 5 were female. One animal was bilaterally affected and left and right eyes were affected in equal proportion. Thorough ophthalmic examination was carried out in all the cases with bilateral evaluation of menace response, palpebral reflex, dazzle reflex, pupillary light response, Schirmer tear test, Fluorescein dye test, ocular tonometry, direct and indirect ophthalmoscopy, antibiogram of corneal swabs, corneal impression cytology and slit lamp biomicroscopy. Twelve corneas selected were randomly divided in to two groups, Group I and II, consisting of six corneas each for surgical treatment. Under general anaesthesia, corneal grafting was carried out in Group I whereas Group II corneas underwent single time intra-operative application of topical mitomycin-C for 2 minutes before corneal grafting with decellularised and gamma irradiated bovine omentum. All the corneas were protected by temporary tarsorrhaphy which was retained up to day 7 and observations viz. corneal oedema, neovascularization, extend of pigmentation, scarring and corneal clarity were serially recorded on day 7, 14, 21 and 60 post operatively. All the corneas in Group I showed negative FDT by day 7, but in Group II, complete epithelialization was obtained by day 7 in 3 corneas and rest showed negative FDT by day 14 post grafting. Corneal oedema subsided by day 14 in all the cases and corneal neovascularization was at the peak on day 7 and day 14 post-grafting which regressed completely by day 60. Corneal clarity had improved gradually throughout the observation period. None of the corneas developed vision impairing corneal melanosis or scarring except one in Group I. Alteration in shape of pupil occurred as a complication of iridectomy in two cases. From the present study it was concluded that re-epithelialization of cornea was enhanced by corneal grafting with decellularised bovine omentum and application of mitomycin-C was effective in controlling corneal fibrosis.EnglishThesis