Naresh, PonnamSubash, RamaduguSahu, Gouri ShankarTripathy, PradyumnaReddy, K. MadhavAcharya, Gobinda Chandra2022-02-112022-02-112021https://krishikosh.egranth.ac.in/handle/1/5810182137Th-6579The present investigation entitled „Evaluation of advanced breeding populations and validation of molecular markers for bacterial wilt resistance in chilli (Capsicum annuum L.)‟ was conducted with objectives of identifying advanced breeding lines with bacterial wilt resistance and validate molecular markers associated with the resistance. Screening of twenty two selected RILs of Anugraha × CM334 through artificial challenge inoculation was carried out and 14 RILs were found to be highly resistant, 4 were resistant, 2 were moderately resistant and 2 were moderately susceptible. And among 242 F4 families of IIHR-B-HP-130×CM334 screened, 28 F4 families were found to be highly resistant followed by 23 families as resistant. And most of the families (164 families) were found to be susceptible to extremely susceptible indicating that resistance is recessive in nature and susceptibility is dominant over resistance. Further evaluation of the selected RILs and F4 families for yield & yield attributing traits were carried out. Analysis of variance revealed highly significant variation for different traits studied in both the populations. PCV value which was greater than GCV was observed among nine characters of the both populations indicating the effect of environment in genotypic expression of traits studied. High GCV, high heritability and high genetic advance as parent of mean were observed for most of the traits in both the populations indicating additive gene action governing the traits. Further through molecular analysis two SSR markers (CAMS 336 and Hpms034) were identified to be associated with bacterial wilt resistance through bulk segregant analysis (BSA) and individual plants screening of selected F4 families and RILs of IIHR-B-HP 130 x CM334 and Anugraha x CM334 respectively. Further reported QTL Bw1 linked marker CAMS 451 was validated and found to be associated with resistance. These markers have to be further extended to large population to confirm their association to use in marker assisted breeding.EnglishThesis