Gutti, Jishnu RaoJustin William, BGeorge, Ravi SundarKannan, TA, et al.TANUVAS2020-05-302020-05-302018http://krishikosh.egranth.ac.in/handle/1/5810146724TNV_JSCRT_2018_8(10)1-5Objective: The study was conducted to evaluate the effect of stress induced by general anesthesia and surgery on the peripheral mobilization of stem cells in horses. Methods: The study was conducted in 12 horses that reported for elective surgical procedures warranting general anesthesia and were randomly divided into group I and group II each consisting of 6 horses. The study included evaluation of plasma cortisol level, stress leukogram and mobilization of peripheral blood Hematopoietic Stem Cells (HSCs), Mesenchymal Stem Cells (MSCs)/progenitor cells. Group I horses were pre-medicated with a butorphanoldexmedetomidine- acepromazine combination and further induced and maintained with ketamine-midazolam. Group II horses were pre-medicated with butorphanol-xylazine and induced and maintained with ketamine alone. Results: The change in the pattern of the differential count in terms of stress leukogram was marked when the horses were induced and maintained with ketamine. The mean percentage of CD34 positive cells in pre-anesthesia, during anesthesia and post-anesthesia were 0.13 ± 0.00, 0.31 ± 0.02 and 0.64 ± 0.03 in group I and in group II, 0.12 ± 0.00, 0.30 ± 0.01 and 0.70 ± 0.03 respectively. Similarly, the mean percentage of CD105 positive cells in preanesthesia, during anesthesia and post-anesthesia were 0.01 ± 0.00, 0.01 ± 0.00 and 0.02 ± 0.00 in group I and in group II, 0.01 ± 0.00, 0.01 ± 0.00 and 0.03 ± 0.00 respectively. The Perusal of mean revealed a significant increase in CD34 positive cells in both the groups on the 6th postoperative day and revealed no significant increase in CD105 positive cells in both the groups. Conclusion: Though the stress indicators in terms of plasma cortisol level and stress leukogram were high after sedation and during anesthesia; mobilization of HSCs was appreciated only after 6th postoperative day indicating mobilization was occurring during the reparative and healing period.enArticle