Kumar, AdeshSrivastava, Shalini2022-08-242022-08-242020-10-29https://krishikosh.egranth.ac.in/handle/1/5810186633ThesisA total of 20 rhizospheric strains were isolated at NA, King’s B and Jension media. Out of 20, only eight isolates (40 percent) showed antagonistic activity against Fusarium oxysporum f.spp.lentis. All the antagonistic isolates produced diffusible and volatile antifungal metabolites. The isolates (B5) and (B8) showed a maximum antagonism as 77.8% and 70% respectively. All the antagonistic isolates showed PGPR activity such as phosphate solubilization, Zn solubilization, Siderophore production ,Ammonia production, HCN and IAA production. On the basis of their antagonistic and PGPR functionality, out of eight, only two isolates, B5 and B8 were found to be more effective which were selected for ISR against wilt of lentil and characterized B5 and B8 strains by 16SrRNA molecular primer. Strain B5 and B8 identified as Bacillus safensis and Bacillus licheniformis respectively. The effect of these two bio-agents (Bacillus safensis and Bacillus licheniformis spp.) against wilt phyto-pathogen (Fusarium oxysporum f.sp.lentis ) lentil (Lens culinaris Medik.) was determined. Two varieties of lentil as resistant (IPL-316) and susceptible (K-75) were taken for the experiment.EnglishThesis