Thilagavathi, K.Sivaseelan, S.Balasubramaniam, G.A.Balasubramaniam, A.Arulmozhi, A.Madheswaran, R.TANUVAS2017-09-012017-09-012017http://krishikosh.egranth.ac.in/handle/1/5810030167Mycoplasma gallisepticum is the most important and infectious mycoplasmosis. It causes severe economic losses to poultry farmers in Namakkal district of Tamil Nadu. Sixty five commercial layer flocks with lesions suggestive of Mycoplasma gallisepticum were investigated during the study period of May 2014 to April 2015. The samples such as choanal cleft swab, tracheal swab, trachea, lungs and air sacs were inoculated into frey’s broth medium and these were subjected to polymerase chain reaction and was confirmed using species specific primer. Out of these 65 farms, 36 farms were positive for MG. it is concluded that, PCR assay for the detection of Mycoplasma gallisepticum infection is good and rapid technique for diagnosis. So it is way to decrease the economic losses to poultry industry.en-USVeterinary ScienceVeterinary PathologyArticle