Singh, M. P.KURMI, DHRUBA JYOTI2017-06-202017-06-202014http://krishikosh.egranth.ac.in/handle/1/5810022060INFLUENCE OF ANTIOXIDANTS, REDUCED GLUTATHIONE AND VITAMIN E ON MICROSCOPIC AND OXIDATIVE STRESS PARAMETERS OF FROZEN THAWED CHHOTANAGPURI RAM SEMENThe result of the present study shows that addition of vitamin E and reduced glutathione in Tris freezing medium improved the percentage of sperm motility, live sperm, plasma membrane integrity, acrosomal integrity and DNA integrity in the frozen thawed semen compared to the Tris control as freezing medium. 2mM Vit E added in Tris dilutor appeared to be the best. Thus on the basis of the present result 2mM Vit E and 5mM GSH may be recommended for supplementation in Tris freezing medium for freezing of Chhotanagpuri ram semen. However further study on large population is required for validation of the result.en-USnullThesis