Savaliya, F. P.THAKER, MITULKUMAR DHANSUKHRAI2018-05-252018-05-252009http://krishikosh.egranth.ac.in/handle/1/5810047535Two hundred and fifty pullets of Synthetic White Leghorn were housed at 16 weeks of age in individual California cage system at Department of Poultry Science, College of Veterinary Science and A.H., Anand. Various principal production traits AFE, Feed consumption during 51-60 weeks, Egg production during 51-60 weeks and up to 72 weeks, BW20, BW40, BW64, BW72, EW32, EW40, EW64 and EW72 were recorded on individual birds. On the basis of feed consumption and egg production during 51-60 weeks the birds were categorized i.e., High feed consumption and High egg production (HH), Low feed consumption and High egg production (LH), High feed consumption and Low egg production (HL) and Low feed consumption and Low egg production (LL) groups. From each group 10 birds were utilized for the present study. Blood DNA was extracted from these 40 birds by John's method with some modification. Chicken gene specific primers were used for amplification of IGF-I, IGF-II, GH and TGF-pa genes loci. Amplified PCR product of IGF-I, IGF-II, GH and TGF-P3 genes were digested with Hinf-I, Hin I-II, Msp-I and Bsel-I RE, respectively for RFLP analysis. Polymorphic pattern were found in IGF-I and IGF-II genes, while for TGF-Ps gene all the samples were monomorphic. MSP-I restriction enzyme didn't cut amplified PCR product for GH gene, which might be due to mutation in line. Genotypic frequency of AA, BB and AB genotypes were 0.10, 0 and 0.90 in HH, 0.40, 0.30 and 0.30 in LH, 0.30, 0.10 and 0.60 in HL, 0.70, 0.20 and 0.10 in LL groups, respectively for IGF-I gene. Genotypic frequency of AA, BB and AB genotypes were 0.70, 0.10 and 0.20 in HH, 0.20, 0.10 and 0.70 in LH, 0.60, 0.10 and 0.30 in HL, 0.30, 0 and 0.70 in LL groups, respectively for IGF-II genotype. In TGFPa gene only LL genotype was observed with allelic frequency of L and B alleles being 1.0 and 0 respectively. Significant (p<0.05) difference was observed between IGF-I genotypes and EN51.60. AB genotype had significantly (p<0.05) higher EN51.60 as compared to AA and BB genotypes, suggesting in heterozygous condition birds had given more egg production. IGF-II genotypes were found to be significant (p<0.05) for BW64. AA and AB genotypes differed significantly (p<0.05) as compared to BB genotype for BW64. IGF-I and IGF-II genotypes were found to be non significantly different for TEN72, BW at 20, 40, 72 weeks, EW at 32, 40, 64, 72 weeks, FC/egg51-60 and AFE. In IGF-I Group X Genotype interaction was significant (p<0.05) only for DFC51-60, where as it was non significant for all traits in IGF-II. In IGF-I group effect was found to be significant for EN51-60, TEN72, FC/egg51-6o, BW40, EW (at 32, 64, 72 weeks), where as it was non significant for other traits. In IGF-II group effect was significant (p<0.05) for DFC51-60, EN51-60, TEN72, FC/egg51-60, BW (at 40 and 64 weeks) and EW (at 40, 64, 72 weeks), while it was non significant for other traits.enANIMAL GENETICS AND BREEDINGA STUDYThesis