Rajora, V.S.Mohd. Reyaz2018-11-012018-11-012008-05http://krishikosh.egranth.ac.in/handle/1/5810082649The present course of investigation was carried out with the objectives to standardize minimum inhibitory concentration (MIC) of garlic extract against bacterial mastitogens and to evaluate intramammary infusion of aqueous garlic extract in the treatment of mastitis in cows. The over all prevalence of mastitis in crossbred cows of varying parity and stages of lactation maintained at Instructional dairy farm, GBPUA&T, Pantnagar (Uttarakhand) was recorded to be 11.86% with more frequent involvement of hind quarters. The occurrence of mastitis was observed to be more upto 60 days post calving and in cows of fourth lactation. The flakes were observed in 75%, pus in 20.83% and watery milk in 4.17% cases. The MIC of crude garlic extract was found to be between 12.5 to 25 mg/ml and garlic in a concentration of upto 100 mg/ml gave clear zone of inhibition against bacterial mastitogens. Twenty four cows showing clinical mastitis were randomly divided equally into three groups for therapeutic evaluation of intramammary infusions of crude garlic extract at 200 mg/ml and 100 mg/ml concentrations and Pendistrin-SH in groups I, II and III, respectively. Besides mild to severe swelling, local pain, warm to hot and firm to hard consistency of udder, presence of the flakes (75%), pus (20.83%) or watery milk (4.13%) was observed in cases of clinical mastitis included in the present study. Affected quarters had significantly higher somatic cell counts (SCC), pH and chloride levels and lower values of milk compositional profile viz. fat, total solids and solids-not-fat in comparison to normal healthy quarters. Biochemical characterization indicated Staphylococcus spp., Streptococcus spp. and Escherichia coli in 11 (45.83%), 5 (20.84%) and 8 (33.33%) milk samples from mastitic quarters. Intramammary infusion of crude garlic extract at the concentration of 200 mg/ml showed 12.5% therapeutic efficacy with resolution in 25% and improvement in 75% cases in terms of swelling, pain, warmness and consistency of the udder. The recoveries in the groups treated with intramammary infusion of garlic extract at 100 mg/ml concentration and Pendistrin-SH were observed to be 50% and 75% respectively with significant improvements in chloride contents, pH and SCC and resolution of clinical manifestations. Crude garlic extract cured 100% and 50% cases infected with Escherichia coli at the concentrations of 100 mg/ml and 200 mg/ml respectively, but slight irritation was observed with crude garlic extract at the concentrations of 200 mg/ml. The Pendistrin-SH was found to cure all the cases except infected with Escherichia coli. Post therapy disappearance of flakes was recorded in all the recovered cases. Crude garlic extract at 100 mg/ml concentration was found to be an effective alternative for the treatment of clinical mastitis infected with Escherichia coli.ennullThesis