Bhardwaj, Rachana D.Dhir, Muskaan2022-12-042022-12-042021Dhir, Muskaan (2021). Biochemical characterisation of barley (Hordeum vulgare L.) mutants with high tiller number (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.https://krishikosh.egranth.ac.in/handle/1/5810190340Sixteen barley mutants with high tiller number along with the check variety (RD 2552) were evaluated for yield and nutritional quality parameters. The average contents of starch, total soluble sugars, β-glucan, total proteins and amino acids were reported to be 561.5 mg/g, 34.27 mg/g, 42.94 mg/g, 148.6 mg/g and 8.62 mg/g respectively. Six mutants namely BM91169, BM91-170, BM91-175, BM91-190, BM92-203 and BM99-238 possessed higher amount of starch. BM91-190 and BM92-215 were having higher content of total soluble proteins. Six barley mutants (BM90-131, BM91-168, BM92-215, BM99-238, BM-249 and BM-250) showed minimum amount of anti-nutritional factors. BM102-249 possessed highest antioxidant potential in terms of total flavonoids, total phenols and tocopherol. Zinc and iron contents were found to be maximum in BM99-238 and BM91-193 respectively. BM90-127, BM99-238 and BM92-215 depicted maximum thousand grain weight along with maximum positive characters, and therefore these mutants were selected for studying sucrose starch metabolism. Sucrose content was found to be maximum in the mutant and check variety during initial stages of grain filling which can be partially attributed to the enhanced Sucrose phosphate synthase (SPS) activity during these stages. Starch accumulation was observed to be maximum in the grains at 40th Days after anthesis (DAA). BM99-238 contained high amounts of nutritional factors such as starch, total fructose, β-glucan, total flavonoids and zinc and low antinutritional factors. It also accumulated maximum starch content during various stages of grain development. Therefore, this mutant may be considered as nutritionally good and may be employed in breeding programmes to develop potential food crop and feed sources.EnglishThesis