Pushpinder Kaur2017-04-282017-04-282013http://krishikosh.egranth.ac.in/handle/1/5810010241Production potential of ethanol, 5-hydroxymethylfurfural and its derivatives from sugarcane bagasse was evaluated. Chemical composition of the bagasse sample was found to be 47.1% cellulose, 29.2% hemicellulose and 11.2% lignin. Different chemical pretreatments of sugarcane bagasse with 2% H2O2, 1% NaOH and 2% H2O2 +1%NaOH solutions each with pH 11.5 and 13 for 24, 48 and 72 hrs followed by acid saccharification with 0.8 and 1.0 M H2SO4 for 50 minutes and fermentation with Saccharomyces cerevisiae var. ellipsoideus were carried out. Maximum weight loss in alkaline pretreatment (45.4%) was obtained by treatment of bagasse with 2% H2O2 +1%NaOH solution at pH 11.5 for 72 hrs. Maximum amounts of reducing sugars i.e. 528.77 and 511.66 mg/g were produced by saccharification with 0.8M H2SO4 (50 min, 15 psi) of (2% H2O2+1%NaOH; pH 11.5,72 hrs) and (2% H2O2+1%NaOH; pH 11.5, 48 hrs) pretreated bagasse, respectively. The bagasse samples pretreated with (2% H2O2+1%NaOH; pH 11.5, 72 hrs), saccharified with 0.8MH2SO4 produced maximum amounts of ethanol (30.5 ml/ 100g pretreated bagasse) after fermentation for 72 hrs. Acid catalyzed dehydration of fructose was carried out to produce 5-hydroxymethylfurfural which on oxidation with KMnO4 yielded 2,5-furandicarboxylic acid. The TLC, IR and 1H NMR spectra confirmed the formation of 5-hydroxymethylfurfural and 2,5-furandicarboxylic acid. The production potential of 5-hydroxymethylfurfural from sugarcane bagasse was found to be 0.216 g/g bagasse. Dimethyl 2,5-furandicarboxylate and diethyl 2,5-furandicarboxylate were obtained by esterification of 2,5-furandicarboxylic acid which were confirmed by TLC, IR and 1H NMR data.ennullThesis