Shanas, S PErra HarishaKAU2020-08-012020-08-012019174759http://krishikosh.egranth.ac.in/handle/1/5810150271PGThe study entitled “Barcoding and biosystematic studies on hymenopteran pollinators of cucurbitaceous vegetables” was conducted during the year 2017- 2019 at the Department of Agricultural Entomology, College of Agriculture, Vellayani with an objective to study the diurnal activity and dynamics of hymenopteran pollinators of cucurbitaceous vegetables and to explore their morphological and molecular diversity. To determine the composition and relative abundance of different hymenopteran pollinators visiting the flowers of five selected cucurbitaceous vegetables viz., culinary melon (Cucumis melo var. acidulus), bitter gourd (Momordica charantia L.), ash gourd (Benincasa hispida Thunb. and Cogn.), pumpkin (Cucurbita moschata L.) and ridge gourd (Luffa acutangula (Roxb.) L.) collections were made throughout the blooming period in Thiruvananthapuram and four other districts of Kerala viz., Kollam, Pathanamthitta, Alappuzha and Kasaragod from 06:00 h to 18:00 h of the day with a cone type hand net. Among the above mentioned vegetables, culinary melon was selected for detailed study on diurnal activity at College of Agriculture, Vellayani. The study on composition and relative abundance of hymenopteran pollinators revealed that, A. cerana indica was the dominant pollinator in culinary melon (42.51 %), pumpkin (38.76 %) and ridge gourd (35.16 %) whereas, T. travancorica was the dominant pollinator in bitter gourd (31.86 %) and ash gourd (33 %). Observations on diurnal activity were carried out at College of Agriculture, Vellayani in culinary melon during two crop seasons for 3 weeks at weekly intervals. For foraging rate, the number of flowers visited by each bee for 1 minute and for foraging speed, time spent by each bee per flower were observed respectively. During two seasons, the foraging speed of A. cerana indica, T. travancorica and Halictus sp. was found to be highest during 10:00-11:00 h (10.61 and 10.63, 11.23 and 11.46, 10.26 and 10.40 seconds) respectively. The foraging speed of C. hieroglyphica and Lasioglossum sp. was found to be highest during 09:00-10:00 h (9.02 and 9.11, 11.06 and 11.30 seconds) respectively. The foraging rate of T. travancorica, C. hieroglyphica and Lasioglossum sp. was found to be highest during 10:00-11:00 h (9.16 and 9.23, 4.83 and 4.85 flowers/m2/5 min) respectively. The foraging rate of A. cerana indica and Halictus sp. was found to be highest during 11:00-12:00 h and 09:00-10:00 h (10.60 and 10.88, 4.03 and 4.13 flowers/m2/5 min) respectively. Samples which were unidentified through morphological characterization were given for DNA barcoding. The sequence of 2 samples viz., Tetragonula sp. nov.1 and T. travancorica were obtained. Among these, new species (Tetragonula sp. nov.1) of stingless bee, based on adult worker specimen is described. Differences in morphology and genetic analysis based on partial sequences of the mitochondrial COI gene barcode region support the recognition of the new species. The above results revealed that A. cerana indica was dominant in culinary melon, pumpkin, and ridge gourd and T. travancorica was dominant in bitter gourd and ash gourd. The foraging speed during two seasons in the descending order was T. travancorica > Lasioglossum sp. > A. cerana indica > Halictus sp. > C. hieroglyphica. The foraging rate during two seasons in the descending order was A. cerana indica > T. travancorica > C. hieroglyphica > Lasioglossum sp. > Halictus sp. Among the pollinators, five species viz., P. phalerata phalerata, C. annulata annulata from ash gourd, M. disjuncta from bitter gourd, C. simillima, and C. unimaculata javanica from culinary melon were reported for the first time pollinating cucurbitaceous vegetables. Tetragonula sp. nov.1 of stingless bee, collected from pumpkin flower, is the new species report from the study and it is morphologically characterised.ennullThesis