Palanisammi, ASatheshkumar, STANUVAS2020-02-282020-02-282019-12http://krishikosh.egranth.ac.in/handle/1/5810143952TNV_35thVCRI_CA_Dec-2019_RBTO-24The objective of the study is to analyze the alterations in the protein profile of follicular cells in normal cyclic and acyclic anoestrus water buffaloes. Ovaries from sexually mature buffaloes were collected from abattoir and categorized into two groups viz., i. Cyclic and ii. Acyclic. In both the categories of ovaries, the follicular fluid (FF) was aspirated from all large follicles (> 9mm diameter) and small follicles (< 9 mm diameter) separately. FF was centrifuged and four categories of pelleted granulosa cells (CSG and ASG: Granulosa cells of small follicles in cyclic and acyclic groups, respectively; CLG and ALG: Granulosa cells of large follicles in cyclic and acyclic groups, respectively) were subjected for protein analysis by standard SDS-PAGE method. A total of 10 aliquots were studied in each category. A total of 30 and 18 bands (from 7.1 to 209.0 kDa) were observed in CSG and CLG categories, while 16 bands in each of the ASG and ALG were recorded. Majority of the proteins were in the range between 40 to 120 kDa in both the groups. It was observed that 40 per cent of bands present in the CSG group were not observed in CLG group. On the contrary, almost all the proteins found in ASG group were retained in the ALG group. It can be inferred that a group of small molecular weight granulosa cell proteins, correlating to IGF/IGFBP system, play a key role in providing a favorable proteo-genomic environment in the early stages of follicular development. Under the gonadotropin stimulus, these proteins might decrease in concentration and release the sequestered metabolic factors during final stages of follicular development enabling the attainment of dominance. In acyclic animals, retaining of such proteins in the large follicles indicated the non-availability of metabolic factors needed for final maturation of follicles.enVeterinary ScienceOther