Dr. R. G. BijurkarPOORNIMA2024-09-132024-09-132024https://krishikosh.egranth.ac.in/handle/1/5810214471The present study was conducted to compare the efficacy of the oocyte quality and recovery rate between slicing, aspiration and dissection method and to examine the potential effects of various vitrification carriers namely Conventional straws, Open pulled straws and Cryolock on the meiotic maturation of immature goat cumulus oocytes. Firstly, recovery of oocytes by three retrieval methods viz., slicing, aspiration and dissection followed by grading into Grade I, Grade II and Grade III was carried out. Further, Grade-I oocytes from various retrieval techniques were vitrified using different cryodevices (G-I CSS, G-II OPS, G-III Cryolock and G-IV Control without any vitrification). The morphological assessment for structural integrity was carried out. Further, the maturation rate of morphologically normal COCs was assessed by percentage mean of COC expansion (degree 1 and 2) and polar body extrusion. The mean oocyte recovery rate per ovary and mean yield of Grade I and II oocyte was significantly (P<0.05) higher in slicing as compared to aspiration and no significant difference (P>0.05) with dissection method. The percentage of damaged COCs in CSS vitrification technique was higher (P <0.01) than OPS and cryolock vitrification technique. The maturation rate showed no significant difference among the experimental groups. In conclusion, slicing was found to be the more effective method for oocyte recovery. Open pulled straw vitrification method offered better protection from cryoinjuries. However, all three cryodevices tested exhibited similar efficacy in promoting oocyte maturation post-vitrification.EnglishThesis