Keshari, NishiS, SETHU LAKSHMI2023-02-012023-02-012022M/NEMA/093/2020-21https://krishikosh.egranth.ac.in/handle/1/5810193049A random survey was conducted in Pusa, Harpur and Bhuskaul village of Samastipur district in Bihar in search of EPNs and found that out of 113 samples collected, four samples were having entomopathogenic nematodes making their total frequency as 3.53 % with Harpur having lowest frequency then Pusa with 1.96 % and with height frequency of 30 % from Bhuskaul. Out of four isolates three were entomophilic nematodes (Acrobeloides spp.) and one isolate of entomopathogenic property (Steinernema spp.) and these isolate were named as BBu1, BBu2, BPm and BBf respectively which were morphologically and molecularly identified using modified CTAB method (Cubero et al., 1999) and the acquired sequence was submitted to NCBI BLAST and further identity was confirmed. The three Acrobeloides spp. isolates are entomophilic in nature and are local to Bihar, thus additional research into the bacteria associated with them is necessary. If bio-efficacy studies are conducted, these nematodes may be used to manage the target crop insect pests in this area. However, further research is needed to understand their life cycle, host range, virulence potential and ability to survive in extreme conditions.EnglishThesis