Sathyabama, TSabir SalimAdarsh Ram, et al.TANUVAS2020-02-282020-02-282019-12http://krishikosh.egranth.ac.in/handle/1/5810143954TNV_35thVCRI_CA_Dec-2019_RBTO-26The present study was carried out to ascertain the presence of Matrix Metalloproteinases (MMPs) enzyme activity in neat semen samples of Murrah breed of buffalo by gelatin zymography and the results were compared with the neat semen samples of exotic cattle breed. In gelatin zymography, the presence of one band at 92 kDa of MMP-9 and 72 kDa of MMP-2 were observed in all the samples. The catalytically active lower molecular weight MMPs were observed around 72 kDa. The activity of MMP-2 was 3-4 times higher than that of MMP-9.The activity of MMP-9 (92KDa) and MMP-2 (45KDa) were observed in Murrah buffalo which was absent in the exotic cattle breed. It was concluded that the overall activity of MMPs was at least two fold greater in buffalo breed compared with the activity of MMPs in Holstein Friesian semen samples. Further, it was inferred that purification and characterization of the species specific enzymes and their effect on semen samples could be correlated to use them as an additive in frozen semen samples, as MMP played extensive role in various physiological processes viz., fertilization, ovulation, milk secretion.enVeterinary ScienceOther