RAHMAN, S. JJAYA KUMARI, G. J2016-08-102016-08-102007http://krishikosh.egranth.ac.in/handle/1/71970Investigations were carried out to evaluate certain shelf life determining and other related parameters of the formulations of Metarhizium anisopliae (Metschinkoff) Sorokin. The influence of different carrier materials viz., Bentonite, Attapulgite, Talc and Kaolinite was tested for the shelf life of Metarhizium anisopliae formulation. Similarly, to find out an optimum temperature for an effective storage, four different temperatures viz., refrigerated (5°C), cool temperature (15°C), room temperature (22°C) and high temperature (40°C) were evaluated. On account of packing material, milky white polythene bags, transparent polythene bags, black polythene bags and silver coated polythene bags were tested for their suitability as packing material. Name of the Student : G . J . J A YA KUMARI Title of Thesis : E V A L U ATION AND STANDARDIZATION OF SHELF LIFE DETERMINING PARAMETERS FOR BIO PESTICIDE FORMULATION OF Metarhizium anisopliae (Metchnikoff) Sorokin Degree for which it is submitted : MASTER OF SCIENCE IN AGRICULTURE Major field of study : E N T O M OLOGY Chairman : Dr. S. J. RAHMAN Name of the University : ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY The out come of the studies indicated that among the carrier material, talc based formulations were found to be more suitable with promising conidial concentration/gm and prolonged viability and pathogenicity. As the Days After Formulation (DAF) were increasing there was a general trend of decline in all the above shelf life determining parameters. As far as storage temperature is concerned, refrigerated temperature (5°C) found to be more suitable than the other temperature ranges tested with high conidial concentration /gm, maximum conidial viability and highest pathogenicity towards target pest. Even though cool temperature and room temperature did fare well but only up to 60 DAF. Packing material is an important parameter that influences the quality and shelf life of the formulation. Several types of packing material were tested. Out of these, polythene bags made of milky white packing material were found to be more suitable followed by silver coated polythene bags. Black and transparent polythene bags were found less suitable for this purpose. Higher conidial concentration/gm with promising conidial viability and pathogenicity was noticed when the formulation is packed in milky white polythene bags. Overall results suggested that M. anisopliae formulation made up of talc based powder and packed in milky white polythene material is proved to have better shelf life with quality of the product maintained especially when stored in refrigerated temperature (5°C) or cool temperature (15°C)enEVALUATION, STANDARDIZATION, SHELF, LIFE, DETERMINING, PARAMETERS, BIO, PESTICIDE, FORMULATION, Metarhizium, anisopliae, SorokinThesis