Dr.Felix, SANTONYRAJ, ADr. Cheryl AntonyDr. Ahilan, B2017-06-052017-06-052016http://krishikosh.egranth.ac.in/handle/1/5810017933The koi carp, Cyprinus carpio var.koi belonging to the family cyprinidae is an attractive ornamental fish that has high domestic and export value in ornamental fish trade. In the present study, relative performance (quantitative and qualitative) of Cyprinus carpio var. koi in AMF (aerobic microbial flocculent) and Non-AMF driven Bio secured Raceways and Aerated Lined ponds was investigated. The various water quality parameters viz., temperature, pH, dissolved oxygen, total alkalinity, hardness, calcium, magnesium, ammonia-N, nitrite-N, phosphate-P, carbon- dioxide, total dissolved solids (TDS), total suspended solids (TSS) and transparency were assessed. The qualitative and quantitative parameters of biofloc such as the composition of living organisms present in the non-aerobic microbial flocculent and aerobic microbial flocculent waters and floc volume (FV) were analysed. The growth parameters such as total length, average body weight, length gain, weight gain, food conversion ratio (FCR) and specific growth rate (SGR) were observed at 10 days of intervals. The experiment was conducted for a period of 60 days in lined ponds and 40 days in raceways. Colorimetric analysis was carried out to assess the colour enhancement after completion of 60 days of culture in lined ponds and 40 days of culture in raceways. The following parameters were studied in aerobic microbial flocculent and non- aerobic microbial flocculent driven lined ponds and raceways. The water quality parameters viz., temperature (26.2°C), pH (7.9), dissolved oxygen (6.3mg/l), alkalinity (64.36mg/l), hardness (197.73mg/l), calcium (44.93ppm), magnesium (37.65plpm), ammonia-N (0.093μg-at-N/l)), nitrite-N (0.02μg-at-N/l)), phosphate (0.16μg-at-P/l), carbon dioxide (8mg/l), total dissolved solids (1103mg/l), total suspended solids (290mg/l), transparency (34.9cm) and floc volume (FV) (16.66ml/l) were studied in aerobic microbial flocculent driven lined pond system. Temperature (26.4°C), pH (8.2), dissolved oxygen (6.6mg/l), alkalinity(74.2mg/l), hardness (209.9mg/l), calcium (63.65ppm), magnesium (48.66ppm), ammonia-N (0.16μg-at-N/l)), nitrite-N(0.009μg-at-N/l)), phosphate (0.05μg-at-P/l)), carbon dioxide (4.4mg/l), total dissolved solids (825mg/l), total suspended solids (159mg/l), transparency (61.50cm) and floc Volume (FV)(0.25ml/l) were studied in non-aerobic microbial flocculent driven lined pond system. Of the fifteen parameters studied, five parameters (ammonia, phosphate, TSS, transparency, cone volume) exhibited a significant difference between the two treatments. The water quality parameters viz., Temperature (25.6°C), pH (7.8), dissolved oxygen (5.5mg/l), alkalinity (171.2mg/l), hardness (304.2mg/l), calcium (38.56ppm), magnesium (40.82ppm), ammonia-N (0.06μg-at-N/l), nitrite-N (0.01μg-at-N/l)), phosphate (0.09μg-at-P/l)), carbon dioxide (7.3mg/l), total dissolved solids (964mg/l), total suspended solids (223mg/l), transparency (27.11cm) and floc volume (FV) (20.24ml/l) were studied in aerobic microbial flocculent driven raceway system. Temperature (26.8°C), pH (8.4), dissolved oxygen (6.6mg/l), alkalinity (195mg/l), hardness (320mg/l), calcium (50.35ppm), magnesium (45.08ppm), ammonia-N (0.23μg-at-N/l), nitrite-N (0.008μg-at-N/l)), phosphate (0.04μg-at-P/l), carbon dioxide (2.6mg/l), total dissolved solids (617mg/l), total suspended solids (132mg/l), transparency (61cm) and floc volume (FV)(0.133ml/l) were studied in non-aerobic microbial flocculent driven raceway system. Of all the fifteen parameters studied, five(ammonia, phosphate, TSS, transparency, cone volume) exhibited a significant difference between the treatments. The maximum body weight (g) in AMF treatment rearing lined ponds (5.8) and non-AMF rearing lined pond (4.7) was in 60th day of culture period. The maximum body weight (g) in AMF driven raceways (3.09) and non-AMF raceway (2.38) was in 40th day of culture period. The maximum length increment (cm) in AMF treatment rearing lined ponds (5.9) and non-AMF rearing lined pond (5) was in 60th days of culture period. The maximum length increment (cm) in AMF driven raceways (7.23) and non-AMF raceway (5.05) was in 40th day of culture period. The maximum SGR in AMF treatment rearing lined ponds (4.8) and non-AMF rearing lined ponds (3.4) was in 60th day of culture period. The maximum SGR in AMF driven raceways (4.7) and non-AMF raceway (4.1) was in 40th day of culture period. The maximum FCR in AMF treatment rearing lined ponds (1.0) and non-AMF rearing lined ponds (1.4) was in 60th day of culture period. The maximum FCR in AMF driven raceways (1.2) and non-AMF raceway (1.0) was in 40th day of culture period. The maximum Total carotenoid concentration (TCC) (μg/g wet wt.) in AMF treatment rearing lined ponds (6.8 μg/g wet wt) and non-AMF rearing lined ponds (3.9 μg/g wet wt) was in 60th days of culture period. The maximum total carotenoid concentration (TCC) (μg/g wet wt.) in AMF driven raceways (4.2 μg/g wet wt) and non-AMF raceway (3.0 μg/g wet wt) was in 40th days of culture period. In the present investigation, the biofloc AMF- water consist of i) prtozoans: Amoeba., Euglena sp. Stentor sp., Tintinids sp., Paramecium sp., Cyclidium sp., Peranema sp., Petalomonas sp., ii) microalgae:Chlorella sp., Oscilatoria sp., Spirulina sp., iii) Planktons: Stephanodiscus sp., Coscinodiscus sp., Navicula sp., (Pennate diatoms),Skeletoma sp., Nitzschia sp. (Pennate diatoms), Chaetoceros sp., Cyclotella sp., Rhizosolemia sp., Schroederella sp., Leptocylindrus sp., Prorocentrum sp., Ceratium sp., Cymbella sp., iv) metazoan: Rotifer sp., Daphnia sp., Moina sp., v) Oligochaeta :Nematode or round worm, Chaetonotus sp. and Cyclops sp. were observed in the AMF treatment tank while the non-AMF tank had only Chlorella sp., Coscinodiscus sp. and Chaetoceros sp.ennullThesis