Dr. A. GIRWANIT. Navya Swetha2019-09-112019-09-112016-07-25Sri Konda Laxman Telangana State Horticultural University, Rajendranagar, Hyderabad-30http://krishikosh.egranth.ac.in/handle/1/5810127349The present study was conducted under four experiments for regeneration of capitulum explants in Murashige and Skoog medium supplemented with different hormonal concentrations with an objective to determine the best concentration of growth regulators for culture establishment, shoots multiplication, rooting of in vitro shoots and further to standardize suitable hardening media for establishment of gerbera plants.The present investigation entitled “STUDIES ON TISSUE CULTURE IN GERBERA’’ (Gerbera jamesonii L.) was carried out at “AGRI-BIOTECH FOUNDATION” in PJTSAU campus and College of Horticulture, Rajendranagar, Hyderabad during the year 2015-2016. In the present investigation, an attempt has been made to determine the response of capitulum explants of gerbera for micropropagation through enhanced axillary shoot method in an elite gerbera variety ‘Savannah’. The present study was conducted under four experiments for regeneration of capitulum explants in Murashige and Skoog medium supplemented with different hormonal concentrations with an objective to determine the best concentration of growth regulators for culture establishment, shoots multiplication, rooting of in vitro shoots and further to standardize suitable hardening media for establishment of gerbera plants. In the first experiment, for culture establishment ten treatments were formulated namely MS basal medium (control), MS medium + BAP, MS medium + BAP + IAA, MS medium + BAP + NAA at different concentrations. Among the treatments MS medium supplemented with 3 mg/L BAP + 0.1 mg/L of IAA was found to be the best medium for culture establishment wherein there were maximum number of responded explants (6.00) and took shortest time for primordial emergence (14.66 days). In the second experiment for shoot multiplication in gerbera, seven treatments were formulated with MS basal medium (control), MS medium + BAP + NAA at different concentrations for shoot multiplication. MS medium supplemented 2 mg/L BAP + 0.5 mg/L NAA was found to produce maximum number of shoots (25.00) in shortest time (12.33 days). In the third experiment for standardization of basal media for in vitro rooting , seven treatments were formulated with MS basal medium (control), MS medium + IBA, MS medium + NAA at different concentrations. MS medium supplemented with 2mg/L IBA was best medium for in vitro rooting because it showed highest per cent rooting (83.33%), earlier rooting (19.00) with maximum root length (54.66 mm). In the fourth experiment media was standardized for establishment and acclimatization of regenerated plants and eight treatments were formulated with sand (control), cocopeat, vermiculite and vermicompost individually and in different combinations. In vermiculite + vermicompost (1:1, v/v) medium, survival percentage was maximum (80.00%) with maximum plant height (47.00 mm). Conclusively, it can be suggested that for regeneration of gerbera from capitulum explants, a combination of MS medium supplemented with 3mg/L BAP + 0.1mg/L IAA was best for initial culture establishment while for shoot multiplication in shortest time, MS medium supplemented with 2mg/L BAP + 0.5mg/L NAA was best. Further rooting of in vitro explants, the MS medium supplemented with 2mg/L IBA was found to be the best media. While, vermiculite + vermicompost (1:1) was found to be the the best media for acclimatization of in vitro raised gerbera plantlets.ennullThesis