Raviprasadh, R.Manoharan, S.Kumanan, K.TANUVAS2016-08-082016-08-082013-10http://krishikosh.egranth.ac.in/handle/1/71496Classical swine fever virus Erns gene (681 bp) was cloned into pET22b vector using DH5 a cells and further expression was done by transforming into BL21 (DE3) Codon Plus RIL cells. The recombinant protein expression was conrmed by SDS-PAGE and western blotting methods using both monoclonal and polyclonal antibodies in dimeric form under non-denaturing condition retaining the conformational epitopes. This could be used as a coating antigen for developing discriminatory diagnostic ELISAs for regular sero-monitoring of animals and also during outbreak situations.enArticle