J.N.PandeyDebashis Chakraborty2024-07-052024-07-051997https://krishikosh.egranth.ac.in/handle/1/5810211487A large number of embryos can be produced by IVM-IVF technique using abattoir ovaries for research and commercial purpose at a very low cost. The technique involves collection, maturation, fertilization and early embryonic development in suitable media. Oocytes were retrieved from the follicles of goat ovaries collected from slaughter house. The maximum retrieval of cocytes (72.70%) was obtained by dissection technique followed by 61.89% through aspiration and 53.31% through puncture technique. Highest retrieval (38.53%) of good quality pocytes was found through puncture technique followed by dissection (32.42%) and aspiration (25.74%) technique. The fair quality of oocytes were retrieved maximum through aspiration technique (52.23%) followed by dissection (46.93%) and puncture (41.40%) technique. The number of retrieved oocytes/ovary were 5.41 +0.33, 4.54 + 0.14 and 4.09 + 0.80 by dissection, aspiration and puncture technique respectively. Oocytes were cultured in TCM-199 + 10% EDS (medium 1) and TCM - 199 + 10% EDS + 10 IU/ml each of PMSG and HCG (medium 2). Maturation upto metaphase - II indicated a significantly higher proportion of oocytes 65.56% reaching metaphase II in medium 2 compared to 53.79% at the same stage in medium 1. Oocytes from larger follicles have better chance of embryonic development than their smaller counterpart. The incubation timeEnglishThesis