Fakrudin, BParamesh, H2019-08-142019-08-142003No. of references120http://krishikosh.egranth.ac.in/handle/1/5810121821Genotypic response for in vitro callus induction and regeneration was studied in six-tomato genotypes viz., Pusaruby, Megha (LI5), PKMl, Dharwad local, SI40 and SI42. Different concentrations and combinations of 2,4-D, kinetin and lAA were used to induce callus and plantlet regeneration. Highly significant differences among genotypes and hormonal concentrations were noticed for callus induction and regeneration. A 2,4-D concentration of 2 mg/1 was found to induce highest frequency of good callus among genotypes. Different calli quality parameters were significantly influenced by the genotype, explant and 2,4-D concentration in the medium. Highest regeneration frequency from callus was obtained in MS basal supplemented with 3 mg/1 of kinetin and 0.3 mg/I of lAA. Megha found to be highly responsive for regeneration through callus. However, kinetin @ 5mg/l induced highest frequency of direct plantlet regeneration in cotyledonary leaf and hypocotyl explants which responded differently across genotypes. Sufficient and healthy i/i vitro root induction was achieved on MS basal supplemented with NAA (0.1 mg/1) and lAA (0.3rag/l). A plantlet survival of more than 95% was recorded during hardening process. Agrobacterium EHA 105 carrying pCAMBIA 1301 with uidA (GUS) and hygromycin selectable gene and pBinBtl with cryl Ab along with nptll gene, both driven by CaMV 35S promoter were used for transformation. Different parameters of transformation process were optimized using i« gene. Cefotaxime @ 300 mg/1 was used to eliminate Agrobacterium. A repeatable average transformation frequency of 2.32% was recorded in Megha. PCR assay using nptll specific primers and insect bioassay studies confirmed the transgenic status of Megha. RT-PCR analysis indicated the expression of transgene in tomato leaves.ennullThesis