Dr. S. K. BhavsarGORIYA HARSHAD VAGHAJI2017-10-072017-10-072004http://krishikosh.egranth.ac.in/handle/1/5810032506The present work was planned to study induction of CYP3A and CYP2H1 genes by Reverse Transcriptase polymerase chain reaction (RT-PCR) and Quantitative RT-PCR in Bantam, Bantamized White leghorn and White leghorn chicks. Out of 18 chicks of Bantam, Bantamized White leghorn and White leghorn, 3 from each group were treated intraperitoneal with phenobarbital at the dose rate of 12mg/100gm body weight and control group were treated with same volume of 0.9% normal saline. After 24 hrs of medication, they were sacrificed and liver samples were collected from each bird. Total RNA was extracted from the liver tissue samples using Tri Reagent® based method. The quantity of extracted RNA was assessed spectrophotometrically at 260/280nmenVeterinary PharmacologyAnalysisThesis