Sumitha, PSukumar, KArthanari Eswaran, MTANUVAS2019-12-302019-12-302015-02http://krishikosh.egranth.ac.in/handle/1/5810138963TNV_IJAS_2015_85(2)126-128Ailing and dead rabbits, brought to the Department of Veterinary Microbiology, VCRI, Namakkal, with a history of anorexia, severe respiratory distress, mucus discharge from the nostril, mild enteritis and recumbency few hours before death, were used for the study. Relevant samples were collected and subjected to cultural examination on nutrient and MacConkey media. Klebsiella pneumoniae was isolated in pure culture and identified by morphological, staining, cultural and biochemical characters. Antibiotic sensitivity test showed multi-drug resistance. Inactivated vaccine was prepared by mixing mineral oil, surfactants and Klebsiella pneumoniae culture in the ratio of 6:1:3. Sterility and safety checking tests were carried out and the vaccine was administered to the rabbits. It produced noticeable protection against the disease.enVeterinary ScienceArticle