Singal, H.R.Tomar, Pooja2016-11-212016-11-212007http://krishikosh.egranth.ac.in/handle/1/86589Protein content of 21 mothbean genotypes viz. Mb 18, GMO 01-02, Mb 5, RMO 435, Mb 16, Mb 2, MH 61, Mb 15, RMB 101, RMB 100, RMM 103, GMO 9915, Mb 14, RMB 4-1, GMO 01-04, CZM 04-01, GMO 9910, GMO 002, Mb 10, RMB 106, GMO 009 ranged from 21.2 (GMO 009) to 27.6 (RMO 435) per cent whereas in check varieties the protein content was 22.5 (RMO 40), 21.4 (Jwala), 22.5 (Jadia) and 23.5 (RMO 225) per cent with an average of 22.5 per cent. Seven genotypes viz. GMO 01-02, RMO 435, MH 61, Mb 2, RMB 100, GMO 01-04 and GMO 002 having protein content higher than other genotypes as well as the average check value were used for further investigations. Globulin was found to be major fraction of mothbean seed storage proteins. The protein content of globulins ranged from 56.7 (GMO 01-02) to 60.5 (RMO 435) per cent of total proteins. Glutelin was the second major protein fraction followed by albumins and prolamins contributing 13.9 (GMO 01-04, GMO 002) to 15.2 (MH 61, Mb 2); 10.9 (GMO 01-02, RMB 100) to 12.2 (GMO 002) and 2.4 (MH 61, Mb 2) to 4.3 (RMO 435) percent of total proteins, respectively. SDS-Polyacrylamide gel electrophoresis separated total proteins and their protein fractions viz. albumins, globulins, prolamins and glutelins resolved into 13, 5, 12, 4 and 6 polypeptides of Mr ranging from 11.22 to 100 kDa, 10 to 56.23 kDa, 12.59 to 141.3 kDa, 7.94 to 56.23 kDa and 8.91 to 50.12 kDa, respectively. Free amino acid content of mothbean genotypes ranged between 3.4 to 6.4 mg/100g flour, being maximum in RMO 435 and minimum in RMB 100. Histidine content of these genotypes varied from 1.75 (GMO 002, Jadia) to 2.37 (RMB 100) g/16g N; cysteine from 0.85 (RMO 40) to 2.12 (Mb 2) g/16g N; methionine from 0.83 (MH 61) to 1.74 (RMB 100) g/16g N; isoleucine from 3.92 (RMO 40) to 5.02 (MH 61) g/16g N; leucine from 6.55 (Jadia) to 7.99 (RMB 100) g/16g N; threonine from 3.13 (Jadia) to 4.93 (RMO 435) g/16g N; lysine from 7.5 (RMO 435) to 8.53 (Mb 2) g/16g N and tryptophan from 0.98 (Jwala) to 1.37 (MH 61) g/16g N. The total essential amino acid contents in these genotypes ranged between 26.67 (Jadia) to 32.40 (Mb 2) g/16g N. Data on adequacy of individual amino acids in mothbean proteins revealed S-amino acid (methionine and cysteine) to be first limiting amino acids followed by tryptophan.enMothbean, Vigna aconitifolia, Protein fractions, SDS-PAGE, Essential amino acidsThesis