Saini, Johar SinghGagandeep2019-09-132019-09-132019http://krishikosh.egranth.ac.in/handle/1/5810127611The present study was conducted with the aim to determine the effect of temperature on alpha-amylase gene expression and germination in wheat. The alpha-amylase gene expression of the chromosome 2 specific gene copies was studied over three temperatures i.e. 10°C, 20°C and 30°C using real time PCR. Differential response of genotypes was observed and KSG 09, KSG220 showed maximum gene expression at 20°C, KSG 30 at 30°C and KSG 072 at 10°C correspondingly. The time period 1-2 days after imbibition was found most crucial for studying the molecular basis underlying alpha-amylase gene expression. Also, rise in gene expression was detected till day 5 and followed by decline upto day 8. Evaluation of forty genotypes for response to germination under varying temperature showed that at 29.2°C, germination percentage was significantly higher than 20.1°C and 14°C however; there was no significant difference in germination percentage between 20.1°C and 14°C. Moreover, interaction between genotype and environment was identified as key aspect for selecting heat tolerant wheat genotypes.666666666666ennullThesis