Tewari, AnujNetam, Amisha2022-05-062022-05-062021-11https://krishikosh.egranth.ac.in/handle/1/5810184333Peste des petits ruminants (PPR) is an economically important transboundary disease of sheep and goats caused by Peste des petits ruminants virus (PPRV). In India, the disease is endemic and therefore, Government of India has initiated PPR control program (PPR-CP) under which kids more than 4 months of age are vaccinated. At the same time it is also important to know the seroprevalence of PPR in unvaccinated goats to know the virus circulation. Therefore, this study was conducted to know the seroprevalence of PPR in unvaccinated goats around the Pantnagar region of Uttarakhand and also to understand the virus distribution in the region. In addition, study also included antibody response and kinetics in Pantja goats vaccinated against PPRV. Total 212 serum samples from goats were collected randomly from various villages from three district (Udham singh Nagar, Nainital, and Almora) of Uttarakhand. Serum samples were tested for anti-PPRV antibody by a commercially available kit from IDvet. 41 animals from various villages were found positive with a prevalence rate of 19.33%. At the same time, PPR outbreaks were also reported from the Pantnagar area. Blood, nasal, oral and rectal swabs were collected from the 19 goats suspected/showing clear sign of PPR. RNA was extracted from the swabs and was subject to one step RT-PCR. The amplified PCR product confirmed PPR in 8 goats with a PPRV prevalence rate 42.10%. Two representative swab samples (one pooled swab and one nasal swab) were subjected to virus isolation in Vero cells. Swabs from both goats showed typical cytopathic effect of PPRV in the first passage and led to complete detachment of the cell monolayer in 48-72 hours in comparison to the vaccine strain Sungri 96 which showed complete cytopathic effect in 4-5 days without complete monolayer detachment. Post vaccination antibody response in Pantja goats vaccinated against PPRV varied from 7-10 days and the antibody response was maintained upto 91 days. Hence the present study signifies that PPRV is circulating in the Tarai region of Uttarakhand and there is an urgent need of mass vaccination to increase the herd immunity to substantial level.EnglishThesis