Soni, RavindraRaj, Kishan KumarChoudhary, TapasSaxena, R.R.Khokhar, DharmendraTiwari, Alok2021-12-272021-12-272021https://krishikosh.egranth.ac.in/handle/1/5810179923BIODEGRADATION OF RICE STRAW BY COW DUNG INHABITING BACTERIAL ISOLATESIn the present study, four bacterial isolates (AMB-CD-1, AMB-CD-2, AMB-CD-3 and AMB-CD-4) was isolated from the cow-dung sample. The biochemical study includes catalase test, Citrate utilization test, Urease test, Starch hydrolysis test, Triple sugar iron test and Antibiotic susceptibility test. Out of four isolates only AMB-CD-1 isolate performed the best in all the screening analysis. The results concluded that the AMB-CD-1 culture inoculated straw have low level of cellulose and hemicellulose indicating the degradation of these particles. The isolate AMB-CD-1 was identified as Pseudomonas aeruginosa through Sanger sequencing technique of 16S rRNA gene. The results from FTIR revealed that the Pseudomonas aeruginosa AMB-CD-1 might be useful for fast composting of rice straw. Compost obtained after degradation of rice straw by AMB-CD-1 was evaluated in rice plant. This study concludes that the highest germination percentage, shoot weight and shoot length were found significantly superior in T4 (50% Sand + 50% Compost) whereas the highest chlorophyll content was found in T7 (25% Sand + 25% Soil + 50% Compost) and T1 (100% Sand) was effective in terms of root length. Overall study concluded that Pseudomonas aeruginosa (AMB-CD-1) might be a fast decomposer of paddy straw.EnglishThesis