Kalaivanan, MSaravanan, SPalanivel, KMPonnudurai, GTANUVAS2022-01-272022-01-272019-06https://krishikosh.egranth.ac.in/handle/1/5810181266TNV_HV_2019_58(1)90-92Dogs are continuously exposed to a spectrum of haemoparasitic diseases and the information on the distribution and epidemiology of trypanosomosis in India is still under reported. Dogs brought to Teaching Veterinary Clinical Campus, Veterinary College and Research Institute, Namakkal of Tamil Nadu were screened for the haemoparasite, T. evansi by polymerase chain reaction. The PCR was developed for detection of Trypanosoma evansi in dogs targeting Beta tubulin mRNA gene at 997 bp, which revealed a positivity of 1.33 per cent. The target gene of T. evansi identified was found to possess nearly a complete homology (99.6-99.8%) with that in China and India. The parasite could not be detected by microscopic examination of the Giemsa stained peripheral blood smears. High prevalence was noticed in dogs of less than 1 year age group (2.94%), males (1.85%) and non-descript dogs (2.94%).EnglishVeterinary ScienceArticle