Dubey, S.K.Bahuguna, Ananya2021-11-112021-11-112021-10https://krishikosh.egranth.ac.in/handle/1/5810178013The current work details about the evaluation of the phytochemical composition of essential oil along with different polarity extracts and the biological activities of plant Anaphalis busua collected from hilly areas of Bhowali Uttarakhand at elevation of 1654 m. The essential oil was chemically analysed by GC-MS for its phytochemical composition. Thirty-four compounds were identified and 3 were unidentified by GC-MS comprising 94.75% of total essential oil composition. Isocaryophyllene (14.07%) was the prominent compound of the essential oil and other major components identified were Pogostol (8.21%), δ- Cadinene (6.81 %), and γ- Curcumene (6.09 %). The essential oil shows significant DPPH free radical scavenging activity having IC50 value of 14.286±0.43μL as compared to standard Ascorbic acid which might be due to the presence of isocaryophyllene. Thirty-one compounds were identified constituting 94.95% of total methanolic extract. Mome inositol (31.03%), Vaccenic acid (10.80%) and Palmitic acid (9.82%) were the major compounds. Twenty-two compounds were identified comprising 68.24% of total hexane extract. Tetracontane (19.33%), tetratriacontane (14.14%) and heneicosane (5.10%) were present in prominent amount. Methanolic extract showed potent antioxidant activity in terms of DPPH radical scavenging and metal chelating activity having IC50 value of 81.71±1.334 μg/mL and 11.26±0.005 μg/mL respectively which might be due to presence of mome inositol. Methanolic extract also exhibited potent anti-inflammatory activity with IC50 value of 24.10±0.09 μg/mL whereas Diclofenac potassium salt used as standard had IC50 value of 18.95±0.03 μg/mL. Total phenolic content (TPC), Total flavonoid content (TFC) and Total antioxidant content (TAC) of methanol and hexane extract were also evaluated. TPC, TFC and TAC was higher in methanol extract having 94.86±0.005 mg/gm, 11.11±0.002 mg/gm, and 36.00±0.011 mg/gm, respectively. A. busua thus have potent medicinal properties and if explored further might be potent antioxidant and anti-inflammatory cost-effective agent compared to synthetically derived agents from pharma industries. Furthermore, the locally grown weed can also be used for its anti-inflammatory properties, as it has demonstrated effective anti-inflammatory activities compared to standard Diclofenac which is a marketed drug. Plant extracts and essential oil could be utilised as powerful antioxidants that might be environment friendly and economically cost viable for the pharmaceutical and cosmetic sectors as plant is found as weed in abundance in hilly areas.EnglishThesis