Mahatma, LalitCharles, Mugisa2018-06-152018-06-152012-06MUGISA CHARLES_52677http://krishikosh.egranth.ac.in/handle/1/5810053150Mungbean or Greengram [Vigna radiala (L.) Wilczek] is an important Pulse grown in India is a rich source of protein and Iron. Besides nourishing the people, the cultivation of Mungbean can sustain soils of India by adding Nitrogen through rhizobial symbiosis. However, Yellow mosaic disease (YMD) caused by Mungbean Yellow Mosaic Virus (MYMV) remains the most destructive disease affecting yield potential of mungbean both quantitatively and qualitatively. To assess the incidence of the disease in Navsari roving field surveys were carried out during summer, 20 I I in major mungbean growing areas of Navsari, Gandevi, Jalalpur, Vansda, Chikhli and taluka. The disease incidence ranged from 06.54 to 33.21 per cent. Crop at the first trifoliate leaf stage at NAU farm, Ugat, and Salem villages of Navsari taluka showed 07.84-13.02 per cent incidence. The incidence increased with the crop growth stages. Around 14.78-17.02 per cent incidence was observed in second trifoliate leaf stage at Pepalkhed, Lakhawadi and Vadichota villages of Vansda taluka. Disease incidence of 30.12-33.21 per cent at Sadakpor and Pepal Gabhar villages of Ckikhli Taluka when observed just before the flowering. The symptoms initially appeared as small scattered yellow to golden yellow colour flecks on the infected trifoliate. The scattered symptoms gradually enlarged covering entire leaves. Severity of the symptoms was mOre on newly emerging leaves of the infected plant. peR amplified and sequenced part of DNA-A molecule gave a 1285 bp nucleotide sequence. The sequence has been submitted to GeneBank, NCBI, and was given accession number JQQ04982. Sequence is having one gene on the virus strand and three genes- on the complementary strand. Analysis and comparison of the sequence with the other standard universal ORF of the DNA-A molecule indicated that the amplified fragment have four genes viz., virus coat protein (AV I) gene (partial cds); replication associated protein (AC3) gene (complete sequence): transactivator protein (AC2) gene (complete cds) and replicase (AC!)ennullThesis