SANDEEP HALMANDGESHILPA2023-12-212023-12-212022https://krishikosh.egranth.ac.in/handle/1/5810204085Lumpy skin disease is an infectious, emerging viral disease of cattle caused by Lumpy skin disease virus (LSDV). The present study reports point prevalence of LSD in cattle at Veterinary Clinical Complex, Veterinary College, Bidar in cattle was 18.85 per cent. Based upon the course of clinical signs, early stage and late stage was recorded in 49.56 per cent and 50.44 per cent respectively. The apparent clinical signs observed were skin nodules (100 %), lymph node enlargement (89.82 %), fever (62.39%) and edema (46.90 %). Haematology analysis revealed leukopenia, lymphopenia, macrocytic anaemia in early stage whereas hypochromic microcytic anaemia and thrombocytosis were observed in late stage of the disease. Serum biochemical analysis revealed increase in AST and ALP levels and hypoalbumenia in LSD affected cattle. Detection of LSDV infection using polymerase chain reaction revealed, out of 26 tissue samples 17 (65.38 %) were found positive for P32 and F gene with 192 bp and 472 bp respectively. Histopathology of skin lesions revealed ballooning degeneration, parakeratosis and hyperkeratosis in epidermal cell layer. Dermal cell layer was characterised by vasculitis, infarction, thrombosis, papillary projections with downward hyperplasia and infiltration of inflammatory cells. Intracytoplasmic round/ oval shaped eosinophilic inclusion bodies were observed in the epithelial cells. Phylogenetic analysis revealed that the current study isolates were having 100 per cent nuclear identity with LSDV isolates from Odisha state.Thesis