Patil, Dr. M. J.SHARATH CHAND, H R.2021-05-292021-05-292019-07-23SHARATH CHAND, H R. (2019).Physiological and biochemical characterization of mungbeanRhizobium. Department of plant pathology, Nagpur. Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. M. Sc. 2019. Print. xii, 73p. (unpublished).https://krishikosh.egranth.ac.in/handle/1/5810169386It relates to collection of mungbean root nodules from different locations and isolation of effective strain of Rhizobium. Different biochemical and physiological tests was conducted. The optimum temperature and pH for growth of Rhizobium sp. were recorded at 30°C and 35°C and 7.0. All isolates were tested for their performance under pot culture experiment conducted in net house condition. Isolate Rh-2 was found to be the most effective in enhancing the growth of the plant in all the three parameters.The Rhizobium is nitrogen fixing bacteria which is essential feature of leguminous plants. Increased cultivation of legumes is essential for the regeneration of nutrient deficient soils. The legume-Rhizobium symbiosis has received most attention and bacteria colonize plant cell with root nodules and commonly found in pulses. Total twenty (20) nodule samples of mungbean plants were collected from twenty different locations of Maharashtra. The isolates were identified as fast growing rhizobia (Rhizobium sp.) and slow growing rhizobia (Bradyrhizobium sp.) on BTB medium. Colonies of bacteria depiciting rod shape, white in colour. All Rhizobium isolates showed circular, convex, White, round, glistening, mucoid and opaque colonies, Bradyrhizobium sp. showed Light pink, round, convex, glistening, mucoid colony. In biochemical tests all the fast growing Rhizobium sp. and slow growing Bradyrhizobium sp. were found to be positive for catalase, oxidase, Potassium hydroxide (KOH), starch hydrolysis, gelatin hydrolysis test and nitrate reduction activities. Negative reaction was found in indole test and for hydrogen sulphide production test. Among twenty isolates, fourteen were positive for hydrogen sulphide test and remmainig 6 isolates (Rh-1, Rh-2, Rh-7, Rh-8, Rh-13 and Rh-19) isolates were found negative. The optimum temperature for growth of Rhizobium sp. were recorded at 30°C and 35°C and optimum growth was noticed in pH 7.0. All isolates were tested for their performance under pot culture experiment conducted in net house condition. Isolate Rh-2 was found to be the most effective in enhancing the growth of the plant in all the three parameters such as shoot length, root length and number of nodules of vigna radiata.EnglishVigna radiata, selection.Thesis