S. K. MukherjeeLt Col Kamal Krishna Prasad2023-11-162023-11-161989https://krishikosh.egranth.ac.in/handle/1/58102012981.The present study was conducted on 70 brood mares maintained at Equine Breeding stud, Babugarh, Ghaziabad, Uttar Pradesh. Prior to selection and inclusion of mares in the research programme, detailed reproductive history was obtained from the history sheets and breading records maintained at the stud farm. 2. In order to assess the present reproductive status, all the selected mares were rectally palpated and categorized under seven groups. Group I and II comprised of normal fertile mares in follicular and luteal phases respectively. Similarly the mares with history of reproductive problems/infertility subsequent to last foaling were kept under group III (follicular phase) and group IV (Luteal phase). The pregnant mares were categorized under three groups, early pregnancy (upto day 90, group IV)., mid pregnancy (day 91 to day 180, group VI), and late pregnancy (day 181 onwards, group VII). 3. Blood samples from all the selected mares were collected by jugular venipuncture at 0800 hours taking all aseptic precautions. Serum was harvested and stored at 04°c before analysis. The sera samples earmarked for progesterone estimation were stored at -20°c alongwith 0.01% sodium azide. 4. Progesterone was estimated by amplified enzyme immunoassay: technique. Estimation of enzyme (ALP and LDH), macronutrients (Ca, P and Mg) and micronutrient (Cu) were carried out by following standard procedures.EnglishThesis