Mahatma, LalitDORE R2018-02-232018-02-232017-07http://krishikosh.egranth.ac.in/handle/1/5810041297Study was undertaken to study the Chilli leaf curl virus (ChiLCV) in South Gujarat. High incidence of the disease in the different fields of NAU as well as the adjoining area could be seen during the survey in 2016. ChiLCD manifesting typical leaf curl symptoms were seen in all the areas surveyed ranging from 10-35 per cent. The variation in disease incidence was mainly due to the different stages of the crop at different place and the different management practices followed. In every infected field surveyed, whiteflies were found invariably. However, jassids and thrips were the other insect pests also noticed. The typical symptoms of the disease observed during the investigation were typical leaf curl symptoms with the upward/downward curling of leaves, puckering and reduced size of leaves along with the thickening and swelling of veins. Affected plants grew slowly and became stunted or dwarfed. Fruit, if produced at all, were small, dry and unmarketable. Quality viral DNA was isolated and in PCR a part of DNA-A molecule of~1200 bp was amplified by the Begomovirus specific primers confirming it to be Begomovirus. A 932 bp nucleotide sequence was obtained on sequencing. Analysis and comparison of the sequence with the other standard DNA-A molecule of the Begomovirus indicated that the amplified fragment have two genes viz., virus coat protein (V1) gene and pre coat protein (V2) gene. The sequenced virus showed highest identity 99% with Chilli leaf curl India virus isolate India:Sonipat:TC290:2010 segment DNA-A, [KJ649706.1]. According to the recent criteria for the nomenclature of the virus was considered and named as Chilli leaf curl India virus – India: Sonipat: Navsari: 2016 abbreviated as ChiLCIV-[IN:Son:Nvs:16]. Alignment of the recently sequenced virus during present investigation [ChiLCIV-[IN:Son:Nvs:16] with other 45 different viruses showed the evolution pattern and similarity of the virus with the other Begomoviruses in the world. Different isolates of Chilli Leaf Curl Virus (ChiLCV) align distinctly in separate clad in the phylogenetic tree prepared.ennullThesis