Hazarika, R ASaikia, Ritrisha2024-01-122024-01-122022https://krishikosh.egranth.ac.in/handle/1/5810206205Leptospirosis is a zoonotic disease found in multiple wild and domestic species including dogs. A study on the sero-prevalence of leptospirosis in dogs of peri-urban livestock farms and urban households of Guwahati was carried out from September 2021 to August 2022. The work was envisaged to collect baseline data from respondents by interview method using a questionnaire, to detect the pathogenic leptospires with serological assay and molecular technique and to map the prevalent areas of leptospirosis in dogs in Guwahati. A total of 240 dogs were selected randomly from peri-urban livestock farms (120 dogs) and urban households (120 dogs). The study recorded higher secondary as the main level of education in both peri-urban farmers (40.00%) and urban household owners (60.00%). Majority of the dogs in peri-urban farms were non-descript (98.33%) compared with maximum of descript dogs (60.00%) in urban households. Female dogs were more common in urban households (55.83%) in contrast to maximum male dogs in peri-urban farms (71.67%). Overall, majority of the dogs aged 1-5 years in both peri-urban farms (38.34%) and urban households (44.17%). Vaccination in dogs was mostly followed by urban households (84.17%). Dogs were provided mainly tap water (56.67%) in peri-urban farms compared to with filtered water in urban households (96.67%). The study recorded peri-urban farms (50.83%) to be moderately clean. Practice of disinfection procedure in peri-urban farms recorded 28.33%. History of no abortion was recorded in peri-urban farms (67.50%) as well as in urban households (85.83%) for both livestock and dogs. Dogs from peri-urban farms (79.17%) and urban households (55.00%) were apparently healthy. Most of the farmers and dog owners were not aware of leptospirosis in both peri-urban livestock farms (96.67%) and urban households (84.17%). Leptospirosis of dogs showed an overall 10.42% sero-prevalence in peri-urban cattle and pig farms and urban households of Guwahati. Sero-prevalence was higher in peri-urban farms (15.83%) compared to urban households (5.00%). It was recorded higher in farms and households where farmers and dog owners were illiterate (30.76%) with non-descript (12.65%) as well as in male dogs (12.94%) and in dogs aged up to 1 year (14.54%). Non-vaccinated dogs (18.39%) were recorded with higher sero-prevalence. Higher sero-prevalence was more in farms providing stored water (52.17%), in dirty farms (29.27%) and farms which don‟t follow disinfection practices (20.93%). It was recorded to be higher in livestock farms having the history of abortion (13.52%), and farmers and dog owners those were not aware (10.56%) of the disease. The samples collected from IgG positive and negative samples from blood, which were subjected to PCR for the detection of 16S rRNA and Lipl32 genes of Leptospira showed their presence (8% each).EnglishThesis