Mehta, NareshWatpade, Santosh Ganpat2016-11-162016-11-162009http://krishikosh.egranth.ac.in/handle/1/85770Present study was carried out with the objective to investigate the callogenic response of rapeseed-mustard species to culture filtrate of Sclerotinia sclerotiorum, oxalic acid and to characterize the calli showing tolerance to culture filtrate along with non-selected calli, based on few biochemical parameters. To study the callogenic response of rapeseed-mustard species to S. sclerotiorum, different concentrations of culture filtrate and oxalic acid were added in MS medium supplemented with NAA 1 mg-1+ BAP 1 mg-1. NAA 1 mg-1+ BAP 1 mg-1 was found most suitable for callus induction to all the four species viz., B. juncea cv. RH-30, B. alba cv. Local, B. napus cv. GSH-1 and B. carinata cv. HC-9002. Per cent callus formation in response to S. sclerotiorum culture filtrate and oxalic acid was more in B. carinata cv. HC-9002 than other Brassica species. Reduction in shoot length and total seedling length due to presence of oxalic acid in germination media was also minimum in B. carinata cv. HC-9002 than other Brassica species. In vitro callus screening with S. sclerotiorum culture filtrate and oxalic acid revealed a similar behavior with field reactions IV of the Brassica species to the disease. As well as seedling screening on germination media amended with oxalic acid also showed correlation with field reactions. Since twenty per cent concentration of culture filtrate was lethal to some genotypes except B. napus cv. GSH-1 and B. carinata cv. HC-9002 and 10mM concentration of oxalic acid is lethal for all species, 15% concentration of culture filtrate and 5mM concentration of oxalic acid can be used for in vitro screening for selection of tolerant species. Increase in concentration of culture filtrate and oxalic acid resulted in decrease of fresh as well as dry weights of calli derived from all the species. Oxalic acid also caused reduction in shoot length, root length and total seedling length of rapeseed-mustard seedlings, 10 mM oxalic acid concentration can be used for in vitro seedling screening and shoot length was better parameter of susceptibility. Total soluble sugars and reducing sugars were high after selection with S. sclerotiorum culture filtrate in calli of B. napus cv. GSH-1 and B. carinata cv. HC-9002 but other species showed less sugar contents. Level of phenol and flavanol were high in all the species after selection. Activity of all the three enzymes viz., polyphenol oxidase, peroxidase and catalase increased with response to increase in concentration of culture filtrate.enFungi, Diseases, Planting, Biological phenomena, Concentrates, Enzymes, Acidity, Organic acid salts, Organic acids, Sclerotinia sclerotiorumThesis