Krishna B., DhanushSULAIMAN, ASHNA2022-02-182022-02-182019-08-29https://krishikosh.egranth.ac.in/handle/1/5810182474In this study, efficiency of decellularisation protocols as well as host response of differently decellularised bovine omental scaffolds were evaluated in a rat model. Bile treated omental scaffold (BBO), triton X- 100 treated omental scaffolds (TBO) and un-processed omental scaffolds (UBO) were used during the implantation period along with sham control (SCO). Efficiency of different decellularisation protocols was assessed by DNA quantification, 4, 6- diamidino-2-phenylindole (DAPI) staining, scanning electron microscopy and histological examination. Cellular and tissue response was evaluated semi- quantitatively using ISO-10993-6: 2016 at 7, 14 and 30 days post- implantation (PI). CD4+ and CD8+ T-cells in retrieved implants and spleen were evaluated immunohistochemically to study the graft acceptance of these implants. Proliferation index was also evaluated by Ki67 immunolabelling. The results of this study showed that bile treatment had better decellularisation efficiency than triton X- 100. BBO showed better remodeling response in terms of neovascularisation, fatty infiltration and neocollagenisation than TBO and UBO. At all time periods studied, BBO implant had significantly (p< 0.05) lesser CD4+ per cent and higher CD8+ per cent than TBO and UBO, respectively. UBO showed significantly higher CD4+ per cent at all time periods than BBO and TBO. The CD4+/CD8+ ratio for BBO was significantly (p< 0.05) lower than TBO and UBO. Besides, BBO showed high Ki67 index (p<0.05) than TBO at 30 days PI. These results document that bile as an effective decellularising agent with less tissue toxicity than chemical detergent, triton X- 100. Apart from these, BBO enhanced remodeling potential through neovascularisation, cellular proliferation and neocollagenisation with significantly lower CD4+/CD8+ ratio and higher Ki67 index. Thus, bile treated omental scaffold may be a promising candidate for applications in human and veterinary medicine for improving patient’s life.EnglishThesis