Nair, Dr. B.HOSUR, PRAKASH LAXMAPPA.2021-05-282021-05-282019-07-31HOSUR, PRAKASH LAXMAPPA. (2019). Genetic variability studies in M5 generation of mustard. Department of botany, Nagpur. Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. M. Sc. 2019. Print. xiv, 84p. (unpublished).https://krishikosh.egranth.ac.in/handle/1/5810169358Present study was conducted to estimate between family and within family variances, to estimate genetic parameters and diversity analysis of 10 high yielding advanced mutants using 20 SSR primers. Twenty SSR primers were used to evaluate 10 mutant genotypes of mustard. The PCR amplified products of each primer were resolved on 3% agarose gel electrophoresis.The present study was conducted with the objectives to estimate genetic variability of the selected mutants based on morphological characters and selection of superior mutants from M5 generation of mustard at AICRP on Linseed and Mustard farm of College of Agriculture Nagpur during rabi 2018 in M5 generation. In rabi2018, 26 advanced mutants along with four checks (Bio 902, Pusa bold, Kranti, Shatabdi) were evaluated in randomized block design in three replication. Data were recorded on days to 50% flowering, days to maturity, plant height, number of primary branches plant-1, number of siliqua plant-1, number of seeds siliqua-1, length of siliqua, seed yield plant-1 and 1000 seed weight. Diversity analysis of 10 high yielding advanced mutants was also done using 20 SSR primers. Analysis of variance indicated that the mean square due to between family and within family were highly significant for all traits, revealing the presence of significant genetic variability between the families. Intra class correlation (t) lead the conclusion that each family distinctly differentiated from each other and differences between individuals within a family is large for all the characters. Therefore it was suggested to assign more weightage to σ2f than σ2w for selection in this generation. The genetic parameter analysis revealed the importance of number of siliqua plant-1 and seed yield plant-1 for selection of better individual mutant from the progenies, based on genotypic coefficient of variation, phenotypic coefficient of variation, heritability and genetic advance. In M5 generation 8 high yielding plants from 26 advanced mutants were selected. Twenty SSR primers were used to evaluate 10 mutant genotypes of mustard. The PCR amplified products of each primer were resolved on 3% agarose gel electrophoresis. Out of 20 SSR primers screened during present study, 12 primers viz.,Na14E08, Na10G10, OI11B05, OI12E03, OI10F06, Ni4H05, Ni2E12, Ni2H06, Ni2Co1, Ni4G09b, Ra2DO4 and OI10F09were found monomorphic and eight primers viz., Na12E01, Na10E02, OI10E05, Na12A08, Na12D04, Na12F11, Ra2E12 and Ra2A11 were found polymorphic for the set of selected genotypes. And based on banding pattern dendrogram was generated for better understanding of the diversity among the selected mutants. Eight mutants viz., ACNMM 20, ACNMM 23, ACNMM 17, ACNMM 12, ACNMM 22, ACNMM 1, ACNMM 3 and ACNMM 9 were selected on the basis of yield and some of these mutants viz., ACNMM 23, ACNMM 22, ACNMM 13, ACNMM 14, ACNMM 7 were observed to be diverse from the checks as they appeared in different clades as that of checks. Some of them were found similar to the checks viz., ACNMM 9, ACNMM 4, ACNMM 17, ACNMM 19 to BIO-902 and ACNMM 15 to Kranti as they occupied the same clade as that of the check. These superior mutants will be further evaluated in multilocation trials of superior and diverse genotypes which can be released as variety or used in breeding programme.EnglishMustard mutants, heritability, genetic advanceThesis