Mall, PramodBira, Lawate Bhiva2019-12-122019-12-122006-07http://krishikosh.egranth.ac.in/handle/1/5810137491The experiments were conducted at Apiculture Laboratory, Department of Entomology and some analytical work was done at Food Processing Lab., Department of Post Harvest Processing and Food Engineering, College of Technology, G.B. Pant University of Agriculture & Technology, Pantnagar. Several experiments were carried out pertaining to fermentation of honey with wine yeast Saccharomyces cerevisiae to produce alcohol and purification of propolis with different solvents viz; ethanol, glycol, mustard oil and water. Organism used for fermentation after activation in YPD broth medium. Readings of alcohol production and residual sugar were recorded at pH 3.8, 4.0, 4.2 and natural pH at 24 hrs, 48 hrs, 72 hrs and 96 hrs of time interval of fermentation of honey with respect to various sugar concentrations viz; 7, 9, 11, 13, 15 per cent. Propolis extraction was recorded at different time intervals by using different solvents. In this investigation it was found that alcohol yield increases with increase in sugar percentage up to certain limit, further increase in sugar concentration hamper alcohol production. It was observed that out of all tested pH, pH 3.8 was most suitable pH where maximum alcohol was produced. Out of different honey concentrations, 13 per cent concentration was optimum to get maximum alcohol. This was again obvious that alcohol fermentation declined consistently with decreasing per cent sugar. However, at higher sugar concentration (15 per cent), yeast showed inhibitory effect on alcohol formation. Alcohol production was found at high rate up to 72 hrs of time interval. Overall maximum fermentation efficiency was recorded at 13 per cent sugar concentration at all pH except 4.20. The maximum residual sugar was found at 15 per cent sugar concentration, irrespective of pH and time interval. In case of propolis purification, ethanol found to be most efficient solvent and showed proportionate maximum effect within three days, Propolis extraction efficiency of used solvents were in order: ethanol > glycol > mustard oil > water.ennullThesis