Garg, S.R.Nagaleelavathi, S.P.2017-08-112017-08-112007http://krishikosh.egranth.ac.in/handle/1/5810028560Haemagglutination inhibition test (HI) and serum neutralization test (SNT) were standardized and used to study the seroprevalence of Japanese encephalitis (JE) antibodies in animals in Haryana. Parallel testing of 213 serum samples by both the tests showed that the sensitivity and specificity of HI in comparison to SNT was 96.29% (92.18%-100%, P0.05) and 100%, respectively. Seventyeight (9.8%) of 793 animals were tested positive for JE antibodies. The seropositivity was the highest in buffaloes (12.6%, n=182), followed by pigs (11%, n=163), horses (9.7%, n=185) and cattle (7.2%, n=263). The prevalence was higher in the animals in the rice cultivating areas (10.6%, n= 601) as compared to those in the non-rice cultivating areas (7.3%, n=192). The corresponding respective values for the two areas were 12.5% and 3.7% in pigs, 10.6% and 5.7% in horses, 7.6% and 6% in cattle, and 13.6% and 10.9% in buffaloes. JE antibody positive samples when tested for West Nile virus (WNV) antibody by HI revealed that 53 of 78 samples were positive for WNV too suggesting that both the flaviviruses are co-circulating in the region. Seroprevalence of JE in animals suggested their role in the transmission of infection which can be correlated to the outbreaks of human disease in the state.enThesis