R L, RAVIKUMARBHOSALE LAXMAN VILAS2016-10-252016-10-2508-07-14TH-10769http://krishikosh.egranth.ac.in/handle/1/81787Lack of genic and genomic resources in legumes particularly in chickpea, horsegram, blackgram, redgram and cowpea, is the major limiting factor for genome mapping and marker assisted selection in these crops. In the present study, 94 pairs of novel chickpea SSR markers comprising of 70 genic and 24 genomic SSRs were screened against a set of fourteen chickpea genotypes, among which 37 produced polymorphic bands. The polymorphic information content (PIC) value for both genic and genomic marker is ranged from 0.15 to 0.37. Among the polymorphic markers 2 to 3 alleles were detected for genic and 2 to 5 alleles for genomic SSRs. The power of chickpea SSR to amplify in chickpea and five other pulses viz., black gram, horse gram, soybean, red gram and cowpea were also tested to determine the transferability of chickpea SSR marker. Out of 94 chickpea SSRs 91 were transferrable to horse gram, 87 to red gram, 84 to black gram, 84 to soybean and 83 to cowpea. It is an important addition to the already available genomic resources in these legumes. Among transferable markers a few primers were polymorphic indicating their potential for application in genetic studies and mapping. The molecular diversity analysis using 37 polymorphic markers divided the 14 genotypes into two main clusters. Highest similarity was found between genotype A1 and JAKI 9303. The morphological diversity analysis of chickpea showed significant variation for number of pods per plant and test weight.enchickpeas, genetics, genomes, genotypes, planting, polymorphism, genetic structures, grain legumes, developmental stages, sowingThesis